Digital Gene Expression Analysis of Populus simonii × P. nigra Pollen Germination and Tube Growth.
Identifieur interne : 001920 ( Main/Exploration ); précédent : 001919; suivant : 001921Digital Gene Expression Analysis of Populus simonii × P. nigra Pollen Germination and Tube Growth.
Auteurs : Li-Juan Zhao [République populaire de Chine] ; Hong-Mei Yuan [République populaire de Chine] ; Wen-Dong Guo [République populaire de Chine] ; Chuan-Ping Yang [République populaire de Chine]Source :
- Frontiers in plant science [ 1664-462X ] ; 2016.
Abstract
Pollen tubes are an ideal model for the study of cell growth and morphogenesis because of their extreme elongation without cell division; however, the genetic basis of pollen germination and tube growth remains largely unknown. Using the Illumina/Solexa digital gene expression system, we identified 13,017 genes (representing 28.3% of the unigenes on the reference genes) at three stages, including mature pollen, hydrated pollen, and pollen tubes of Populus simonii × P. nigra. Comprehensive analysis of P. simonii × P. nigra pollen revealed dynamic changes in the transcriptome during pollen germination and pollen tube growth (PTG). Gene ontology analysis of differentially expressed genes showed that genes involved in functional categories such as catalytic activity, binding, transporter activity, and enzyme regulator activity were overrepresented during pollen germination and PTG. Some highly dynamic genes involved in pollen germination and PTG were detected by clustering analysis. Genes related to some key pathways such as the mitogen-activated protein kinase signaling pathway, regulation of the actin cytoskeleton, calcium signaling, and ubiquitin-mediated proteolysis were significantly changed during pollen germination and PTG. These data provide comprehensive molecular information toward further understanding molecular mechanisms underlying pollen germination and PTG.
DOI: 10.3389/fpls.2016.00825
PubMed: 27379121
PubMed Central: PMC4908133
Affiliations:
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Department of Crop Molecular Breeding, Crop Breeding Institute, Heilongjiang Academy of Agricultural SciencesHarbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityHarbin, China; Department of Crop Molecular Breeding, Crop Breeding Institute, Heilongjiang Academy of Agricultural SciencesHarbin</wicri:regionArea><wicri:noRegion>Heilongjiang Academy of Agricultural SciencesHarbin</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Hong Mei" sort="Yuan, Hong Mei" uniqKey="Yuan H" first="Hong-Mei" last="Yuan">Hong-Mei Yuan</name><affiliation wicri:level="1"><nlm:affiliation>Medical Plant Research Center, Economic Crop Institute, Heilongjiang Academy of Agricultural Sciences Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Medical Plant Research Center, Economic Crop Institute, Heilongjiang Academy of Agricultural Sciences Harbin</wicri:regionArea><wicri:noRegion>Heilongjiang Academy of Agricultural Sciences Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Wen Dong" sort="Guo, Wen Dong" uniqKey="Guo W" first="Wen-Dong" last="Guo">Wen-Dong Guo</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences Harbin</wicri:regionArea><wicri:noRegion>Heilongjiang Academy of Sciences Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Chuan Ping" sort="Yang, Chuan Ping" uniqKey="Yang C" first="Chuan-Ping" last="Yang">Chuan-Ping Yang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin</wicri:regionArea><wicri:noRegion>Northeast Forestry University Harbin</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pollen tubes are an ideal model for the study of cell growth and morphogenesis because of their extreme elongation without cell division; however, the genetic basis of pollen germination and tube growth remains largely unknown. Using the Illumina/Solexa digital gene expression system, we identified 13,017 genes (representing 28.3% of the unigenes on the reference genes) at three stages, including mature pollen, hydrated pollen, and pollen tubes of Populus simonii × P. nigra. Comprehensive analysis of P. simonii × P. nigra pollen revealed dynamic changes in the transcriptome during pollen germination and pollen tube growth (PTG). Gene ontology analysis of differentially expressed genes showed that genes involved in functional categories such as catalytic activity, binding, transporter activity, and enzyme regulator activity were overrepresented during pollen germination and PTG. Some highly dynamic genes involved in pollen germination and PTG were detected by clustering analysis. Genes related to some key pathways such as the mitogen-activated protein kinase signaling pathway, regulation of the actin cytoskeleton, calcium signaling, and ubiquitin-mediated proteolysis were significantly changed during pollen germination and PTG. These data provide comprehensive molecular information toward further understanding molecular mechanisms underlying pollen germination and PTG. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">27379121</PMID><DateCompleted><Year>2016</Year><Month>07</Month><Day>06</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Digital Gene Expression Analysis of Populus simonii × P. nigra Pollen Germination and Tube Growth.</ArticleTitle><Pagination><MedlinePgn>825</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2016.00825</ELocationID><Abstract><AbstractText>Pollen tubes are an ideal model for the study of cell growth and morphogenesis because of their extreme elongation without cell division; however, the genetic basis of pollen germination and tube growth remains largely unknown. Using the Illumina/Solexa digital gene expression system, we identified 13,017 genes (representing 28.3% of the unigenes on the reference genes) at three stages, including mature pollen, hydrated pollen, and pollen tubes of Populus simonii × P. nigra. Comprehensive analysis of P. simonii × P. nigra pollen revealed dynamic changes in the transcriptome during pollen germination and pollen tube growth (PTG). Gene ontology analysis of differentially expressed genes showed that genes involved in functional categories such as catalytic activity, binding, transporter activity, and enzyme regulator activity were overrepresented during pollen germination and PTG. Some highly dynamic genes involved in pollen germination and PTG were detected by clustering analysis. Genes related to some key pathways such as the mitogen-activated protein kinase signaling pathway, regulation of the actin cytoskeleton, calcium signaling, and ubiquitin-mediated proteolysis were significantly changed during pollen germination and PTG. These data provide comprehensive molecular information toward further understanding molecular mechanisms underlying pollen germination and PTG. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Li-Juan</ForeName><Initials>LJ</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityHarbin, China; Department of Crop Molecular Breeding, Crop Breeding Institute, Heilongjiang Academy of Agricultural SciencesHarbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Hong-Mei</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>Medical Plant Research Center, Economic Crop Institute, Heilongjiang Academy of Agricultural Sciences Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Wen-Dong</ForeName><Initials>WD</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chuan-Ping</ForeName><Initials>CP</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Front Plant Sci. 2016;7:1056</RefSource><PMID Version="1">27486470</PMID></CommentsCorrections></CommentsCorrectionsList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">DGE</Keyword><Keyword MajorTopicYN="N">Populus simonii × P. nigra pollen</Keyword><Keyword MajorTopicYN="N">differentially expressed genes</Keyword><Keyword MajorTopicYN="N">pollen germination</Keyword><Keyword MajorTopicYN="N">pollen tube growth</Keyword><Keyword MajorTopicYN="N">transcription</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>11</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>05</Month><Day>26</Day></PubMedPubDate><PubMedPubDate 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